As in (E) but with oleate for 6 h. GFP-PLIN1 is now found on peripheral LDs. PLIN1 remains excluded from the LDs covered by endogenous PLIN1. On the right, an anti-PLIN1 antibody stains both endogenous PLIN1 on large LDs (red) and PLIN1 on the peripheral LDs (GFP+red = yellow).
