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(Left) Representative flow cytometric plot showing the applied gating strategy used to identify HLA-A*02:01-NLVPMVATV tetramer−/+CD8+T cells. Only individuals with >150 events within the NLV+gate were considered for subsequent analysis. (Middle) Representative flow cytometric plot showing the applied gating strategy used to identify HLA-DP−/+HLA-A*02:01–NLVPMVATV tetramer+CD8+T cells. (Right) Median percentage (+IQR) of HLA-DP−and HLA-DP+HLA-A*02:01-NLVPMVATV tetramer+CD8+T cells. Shown are results from n= 4 individuals, and each dot represents one biological replicate. Statistical significance was determined using Wilcoxon matched-pairs signed-rank test.
#Bar Plot#Error Bars#Scatter Plot#Flow Cytometry#Gating Strategy#HLA-A*02:01-NLVPMVATV Tetramer- CD8+ T Cells#HLA-A*02:01-NLVPMVATV Tetramer+ CD8+ T Cells#HLA-DP- HLA-A*02:01-NLVPMVATV Tetramer+ CD8+ T Cells#HLA-DP+ HLA-A*02:01-NLVPMVATV Tetramer+ CD8+ T Cells#Wilcoxon Matched-Pairs Signed-Rank Test
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