Sequence logos from the Gr43a subfamily covering the ligand-binding pocket regions; corresponding secondary structure elements indicated above and BmGr9 sequence below. The 21 pocket-lining positions are shaded gray. Black arrowheads mark conserved pocket-facing residues highlighted in (D). Colored circles mark positions assessed by alanine substitutions in BmGr931and highlighted in (E) (red, pocket-lining and <1% activity compared to wild-type BmGr9; orange, pocket-lining and ~10% activity; blue, outside pocket and <1% activity; cyan, outside pocket and ~10% activity; gray, outside pocket and >=40% activity).
