Schematic view of a typical coverage of a genomic region, following targeted NGS. Individual reads are represented by thin horizontal lines and coverage by grey-shaded areas. Since, at the pre-sequencing stage, capturing probes target only specific sequences of the genome (e.g., exons, in blue, in B), coverage tends to be higher for such regions of interest. However, target selection and downstream fragment purification are not 100% efficient, and DNA fragments that lie in uncaptured regions are frequently co-purified along with fragments from targeted areas.
