We independently treated primary fibroblasts from two different individuals in duplicates (four biological replicates) with three different doses (17, 850, and 5,100 ng RNA) of three types of lipid nanoparticle ABE8e (mRNA-1273, BNT162b2, and ALN-18328). We followed the fibroblast populations for 70 h, taking pictures in the Incucyte every 2 h. The lipid nanoparticle ABE8e treatment was applied at the 2 h time point. We assessed the confluency (D)and the number of dead cells (E)estimated by the CytoTox dye. The solid lines represent the mean of each treatment. For simplicity, the error bars (SD) are shown only for the non-treated samples (in black). For more detailed data, please checkFigure S5.
