Relative viable cell counts of T cells expressing indicated sgRNAs after prolonged (3 weeks) chronic D4M.OVA stimulation. Genes with significantly increased cell count after chronic stimulation (Figure 4C), but without proliferation (dis-) advantage (+/− 25% change) under resting condition (Figure S4B), were selected for prolonged chronic in vitro stimulation. Analyzed with one-way ANOVA, followed by a Dunnett post-hoc test from three biological replicates with two different sgRNAs per replicate (n = 3x2).
