vAC formation time course, seeFigure S7for full data. Cells were infected with WT or US33A HCMV at MOI 1. At 24 h post-infection (hpi), both viruses infected a similar proportion of cells (Figure S7B). Left panel: representative images at 48 hpi. Pink arrows: examples of mature vACs. Orange arrows: examples of early/immature vACs. These were counted separately for the purpose of quantification (Figures S7C andS9C). Scale bars: 20 m. Right panel: percentage of infected cells with mature vACs at each time point. Similar results were observed in a second independent experiment (Figure S8). The number of images with infected cells assessed at each time point were: 24 h (n = 21 images with 112 infected cells for WT; n = 22, 115 for US33A); 36 h (n = 31, 122 for WT; n = 21, 146 for US33A); 48 h (n = 21, 240 for WT, n = 27, 241 for US33A); 72 h (n = 22, 205 for WT, n = 21, 188 for US33A); 96 h (n = 25, 389 for WT, n = 27, 321 for US33A). p values were estimated using a two-tailed unpaired t test.***p 0.001,****p 0.0001.
