US33A inhibits lysosomal re-acidification. Left panels: schematics of workflow. Right panels: quantification of lysosomal acidity via LysoTracker staining in HFFF-TERTs expressing US33A or a control vector (top; 400 nM BafA1 for 1 h) or infected with parental or US33A HCMV (bottom; 100 nM BafA1 for 1 h). Values shown are mean SEM (n = 3 independent experiments). p values were estimated using an unpaired two-tailed t test.*p < 0.05.
