Quantification of LysoSensor staining using a microplate reader with excitation at 340 or 380 nm and emission at 527 nm as per previous protocols.31,32Staining with LysoSensor was at 5 M for 10 min. Left panel: HFFF-TERTs expressing US33A or a control vector. Middle panel: HFFF-TERTs expressing the coxsackie virus and adenovirus receptor (HFFF-CARs) infected with adenovirus expressing US33A or GFP (MOI 5 for 72 h). Right panel: HFFF-TERTs infected with WT or US33A HCMV (MOI 5 for 24 h). Values shown are mean SEM (n = 3 independent experiments; each value was calculated from the mean of five technical replicates).
