Quantitation of ZO-1 and PECAM-1 mean fluorescence intensity (MFI) of ten individual representative regions of interest (ROIs). Total ZO-1 expression (upper left), nuclear ZO-1 expression (upper middle), ZO-1 expression in membrane cells adjacent to E2-CHIKV-positive cells (upper right), total PECAM-1 (bottom left), and percentage of positive PECAM-1 (bottom right) in CHIKV-positive cells (E2-positive), CHIKV-negative cells (E2-negative), and uninfected cells. Data are presented as the mean standard deviation. Statistical analyses were performed using the paired t test and one-way ANOVA. Statistical significance is***p < 0.001,**p < 0.01, and*p < 0.05; ns, not significant.
