Percentage of permeability of the BBB model, assessed by quantifying the passage of albumin conjugated to Evans blue dye through cocultures of primary human brain microvascular endothelial cells (BMVECs) and human primary astrocytes. Mock is only cocultures. CCL-2 is cocultures plus CCL-2 at 100 ng/mL. PBMC is peripheral blood mononuclear cells (PBMCs), cocultures, and CCL-2 at 100 ng/mL. CHIKV is PBMC infected with CHIKV at MOI of 0.1 plus cocultures, and CCL-2 at 100 ng/mL. EDTA is coculture and treatment with 4 mM of ethylenediaminetetraacetic acid (EDTA) as a positive control for disruption of the BBB. The data are expressed as a percentage of permeability related to EDTA.
