Bar graphs comparing theKm(left) orkobs(right) values of wild-type (WT) or UBE2R2 acidic tail mutants for neddylated CRL2VHL. Notice that deletion of residues 198 to 203 (Δ198-203) or a “4G” quadruple point mutant (L206G Y207G L210G Y211G) resulted in both a decrease in the apparent affinity of the mutants for the CRL2 complex as well as reductions in the rates of BRD4 neo-substrate priming. See alsoFiguresS6,S7A, and S7B andTables1andS1.
