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Schematic illustrating how fluorescence resonance energy transfer (FRET) is achieved between two fluorophores conjugated to neddylated CUL2-RBX1 and UBE2R2. Excitation of Alexa Fluor 568 on neddylated CUL2-RBX1 produces fluorescence emission that is sensitive to the presence of UBE2R2 conjugated to Alexa Fluor 647.
#Workflow#Illustration#Fluorescence Resonance Energy Transfer#FRET#CUL2-RBX1#UBE2R2#Alexa Fluor 568#Alexa Fluor 647
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