Schematic illustrating how fluorescence resonance energy transfer (FRET) is achieved between two fluorophores conjugated to neddylated CUL2-RBX1 and UBE2R2. Excitation of Alexa Fluor 568 on neddylated CUL2-RBX1 produces fluorescence emission that is sensitive to the presence of UBE2R2 conjugated to Alexa Fluor 647.
