Gel from Scientific Research

Degradation of DyLight 800-labeled, photo-cleaved Ub(n)-FOLD by 26S proteasomes in the presence of the Cdc48-UN complex. Cdc48 was either wild-type (WT) or contained a mutation (E588A) that abolished its unfolding activity. Quantification was done as in (A). The total fluorescence in the lanes was the same within a range of 10%.

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