Gel from Scientific Research

RNA extension assay as performed inFigure 1B. NELF-A mutants were used in place of wild-type NELF-A in the context of the complete NELF complex where indicated. NELF-A truncation includes residues 1–276. NELF-A protrusion mutant includes four-point mutations: R252D, V254R, L257K, D258R. Results are representative of three replicates.

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