Gel from Scientific Research
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Stably transduced RPE1 cells, harboring a doxycycline-inducible HA-p21, HA-p21(ΔPCNA), or EV, were synchronized into G0. Transgenes were induced by doxycycline for 12 h before serum re-addition. G1 cells were treated with H2O2before fractionation into soluble and chromatin fractions (right). PCNA was immunoprecipitated from the chromatin fraction and immunoblotted with co-purified proteins (left). See alsoFigures S12–S14.
#Gel#RPE1 Cells#Doxycycline-inducible HA-p21#HA-p21(ΔPCNA)#EV#G0 Synchronization#Transgenes#H2O2#Fractionation#Soluble Fraction#Chromatin Fraction#PCNA#Immunoprecipitation#Immunoblot#Co-purified Proteins
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