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U-2OS cells were transfected with the indicated siRNAs and synchronized in G1. Cells were then treated and analyzed as in (A). Where indicated, cells were pretreated for 2 h with palbociclib (CDK4/6i) and kept in palbociclib during the 4-h recovery. Lines represent mean values on the plots from 3 independent experiments. The efficiency of protein depletion and CDK4/6 inhibition is shown in the right panel.
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