Bar Plot from Scientific Research

RPE1 cells were transfected with the indicated siRNAs and synchronized in G0. Cells were then nucleoporated with the indicated FM-HCR reporter plasmids and released into serum-containing media. 8 h later, cells (now in G1) were subjected to fluorescence-activated cell sorting (FACS) analysis. DNA repair activity for each siRNA was normalized to siNT, while also correcting for transfection efficiency, and was represented as normalized repair activity. Graphs show average and standard deviation from at least 3 independent experiments. Schematics of the fluorescent reporters are shown next to the graphs. Silencing efficiencies are shown in the right panel.

DOI