RPE1 cells were transfected with the indicated siRNAs and synchronized in G1. Where indicated, cells were treated with H2O2 for 30 min and then allowed to recover for 1.5 h in DMEM supplemented with EdU. The EdU signal was normalized to the untreated siNT samples. Measurements were carried out from 3 independent experiments. Dashed lines represent the median on the plots. Silencing efficiencies are shown in the right panel.
