U-2OS cells stably expressing mCherry- or mNeonGreen-tagged BER proteins or AcGFP-tagged MMR proteins were transfected with siNT- or siRNA-targeting D-type cyclins and subsequently synchronized in G1. Each row in the heatmap shows the recruitment of a BER or MMR protein at micro-irradiation sites that is either higher (red) or lower (blue) in D-type cyclin-depleted cells when compared with control cells. Columns represent standardized recruitment values that were averaged over 15-s intervals. Column label units refer to seconds post-irradiation. The “p” column indicates whether the mean intensity of the normalized difference between control and D-type cyclin-depleted cells was significant over a window of 60 s across the entire 10 min (+p < 0.01). Raw curves are reported inFigure S7.
