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RPE1 cells harboring tagged endogenous proteins (mRuby-PCNA, mVenus-cyclin D1, and p21-mTurquoise2) were micro-irradiated as in Miwatani-Minter and Rona.46DNA damage recruitment dynamics were captured by live-cell imaging. Non-S-phase cells were identified and analyzed as inFigure1A.
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