Gel from Scientific Research
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Left: HEK293T cells were co-transfected with the indicated FFSS-cyclin D1 constructs together with p21-HA or empty vector (EV). Cyclin D1(MV) denotes mutations M56A/V60A. Cyclin D1(HP) denotes mutations M56A/V60A/W63A. Cell lysates were immunoprecipitated with an anti-FLAG resin and immunoblotted. Whole-cell extract (WCE) is from the EV sample. Middle and right: U-2OS cells stably expressing the indicated mAzGreen-cyclin D1 constructs and mPlum-PCNA were analyzed as in (A).
#Gel#HEK293T Cells#FFSS-Cyclin D1#p21-HA#Empty Vector#Cyclin D1(MV)#Cyclin D1(HP)#Immunoprecipitation#Immunoblotting#U-2OS Cells#mAzGreen-Cyclin D1#mPlum-PCNA
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