Microscopy from Scientific Research

Lysosomal damage detected by SPG20 is associated with lipid-packing defects. Cells expressing mCherry fusions of the amphipathic lipid-packing sensor (ALPS) derived from ARFGAP1 or an ALPS mutant where bulky hydrophobic residues are substituted with small hydrophobic residues (SH mutant) were mock or LLOMe-treated and costained for SPG20 and LAMP1. See Figure S8A for LAMP1 staining.

DOI