Quantification of lysophagy assay (C). mKeima fluorescence was assessed at both excitation wavelengths, and the ratio between both intensities was determined. Note that the ratio of red to green-excitable mKeima increases during 6 h chase indicating lysophagy, but this is largely reduced in SPG20 and ITCH-depleted cells. n = 3 (for siSPG20 #3, #4, and #5 n = 1) biologically independent experiments with >30 cells quantified per condition. One-way ANOVA with Tukey’s multiple comparison test. Line indicates mean.
