Triaging of lysosomes into repaired and unrepaired lysosomes. Statistics: (B, D, and E) n = 3 biologically independent experiments, with >20 cells quantified per condition. (F and G) >30 cells quantified for each time point. (B and G) Lines indicate median. (D and E) Error bars, SD. (B) Unpaired t test. (G) One-way ANOVA with Tukey’s multiple comparison test. (D and E) Allosteric sigmoidal fit. Scale bars: 10 μm in (A) and (C) and 5 μm in (F).