NGS of genomic DNA after introduction of the top-scoring sgRNA in the A -> G base-editor screen (D) into the screening cell line, TNFAIP3-/- NF-kB-DCK*NALM-6 cells. The pie chart shows percentage of NGS reads that contained substitutions, and the nucleotide bar graph shows the frequency of individual substitutions at the indicated nucleotides, along with canonical amino acid sequence above and validated mutated amino acid sequence below. Note that the sgRNAs in both panels align with the complementary DNA strand, so substitutions introduced are T -> C and G -> A in this orientation. A naturally occurring A/G SNP (rs2241502) is present in panel (D) as well. Analysis done with CRISPResso2.
