FACS-based competition assay of the indicated NALM-6 reporter cells. The cells were stably infected to produce an MYD88 sgRNA and mCherry or a nontargeting control sgRNA and blue fluorescent protein (BFP). The cells were then mixed 1:99, respectively, and treated with 10 μM BVdU (or DMSO) and 10 μg/mL LPS for 20 days prior to FACS.
