In vitro analysis of KPC-1 engulfment by KCs in the presence of PBS control ( n = 5 experiments), phosphatidylserine blockade (MFG-E8(D89E); n = 3) or actin inhibitor (latrunculin A (lat. A); n = 3). KCs and dying cells are labelled as in f . The open arrow shows engulfing KCs. The closed arrow shows caspase-3/7 cleavage. The plots show the percentage of KCs engulfing Cas-Green KPC-1-tdT cells, and the time from stable interaction between individual KCs ( n = 17 (PBS) and n = 31 (MFG-E8(D89E))) and tumour cells to engulfment and caspase-3/7 cleavage.
