Sum of peak areas for all BBAAs in ileal contents measured by LC–MS/MS (P = 3.1 × 10 −4 ***). f – i , box plots depict first quartile and third quartile with median as centre and min–max values for whiskers, except for outliers calculated as data points ±1.5× interquartile range. Significant differences (P < 0.001***, P < 0.0001****) were determined by two-tailed t -tests. MCA, muricholic acid; UDCA, ursodeoxycholic acid.