Left: First two principal components (PCs) extracted from composite behavior measures for opioid-naive rats (purple), rat 8 (red filled), and all other opioid-exposed rats (teal). Crosses represent corresponding centroids. Right: Mean difference of rat 8 centroid under true labels (red dashed line) compared to bootstrapped distribution under shuffled labeling shows greater similarity of rat 8 to the opioid-naive group. Black dashed lines indicate two-tailed 95% threshold of null distribution. Source data are provided as a Source Data file.
