A representative panel of cells ( n = 90) imaged to ascertain the intensity of red and green fluorophores in 3-h intervals after penning. Cell cycle designation to the left of each cluster was determined by the fluorophore intensity at the time of penning seen in column 1 and denoted by the gray border. Subsequent heatmap columns represent 3 hour imaging timepoints of the same cell. Cell cycle color key circle is below. Models to the right implicate theoretical strand-specific (blue/yellow) distribution of DNA damage (red triangles) depending on the cell cycle phase at the time of UV treatment (timepoint 0).
