CD4 + Jurkat cells were transduced with the CD4 + TIL-derived library of patient OVC190 ( n =1,341 TCRs encoded as 2,900 unique nucleotide sequences). Patient immortalized B cells were engineered to express TMGs encoding all expressed non-synonymous tumor mutations ( n =61) and used to screen the OVC190 TCR library. Dots represent individual TCRs. Codon-diversified replicates of individual TCRs are designated by suffixes 'a–e'. Screen hits and P values were defined as in Fig. 2.
