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Neoantigen specificity of TMG-reactive TCRs was determined by incubating TCR-transduced Jurkat cells with TMG constructs in which indicated mutant minigenes were reverted to their wild-type sequence. T cell activation was evaluated by measuring CD69 expression using flow cytometry.
#Clustered#Bar Plot#Neoantigen Specificity#TMG-Reactive TCRs#Jurkat Cells#TMG Constructs#CD69 Expression#Flow Cytometry
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