NKIRTIL063 immortalized B cells were transduced with TMG constructs encoding the patient’s 200 most highly expressed non-synonymous tumor mutations. TMG B cell lines were combined into five pools of four lines and used to screen the NKIRTIL063 TCR library. MART1 26–35 minigene-expressing B cells were included in TMG pool 3. Dots represent individual TCRs. Fold change represents the relative abundance of TCRs in cultures with the indicated TMG pools.
