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Pooled TCR gene assembly scheme. Gray fragments indicate reusable TRBC1, TRAV_GL or TRBV_GL gene sequences (GL, germline). Arrows indicate 3' of DNA strands. ConnA, TRAV gene-specific connector sequences; ConnB, TRBV gene-specific connector sequences; Zip, TCR-specific barcode sequence. Asterisks indicate reverse complement sequences. The products of individual assembly steps are indicated by circled numbers.

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