Histogram from Scientific Research

z height distribution of particles in a single micrograph. z height is the distance of particles from the fitted plane, which corresponds to spatial distributions along the z axis. The peak of the KDE fitting curve indicates the mode of z heights, while the zero point corresponds to the mean distance. The unimodal distributions of z heights of apo-ferritin, ACE2 and streptavidin particles are distributed in asymmetrical shapes with a long tail when supported on the holey gold grid with two interfaces in contact with the air (purple), or the EM grids with one interface in contact with the air and the other in contact with the supporting film (green), respectively. In some micrographs, z heights of apo-ferritin particles prepared on rGO show bimodal distributions, indicating adsorption at both AWIs and GWIs. 70S ribosome, 20S proteasome, apo-ferritin, ACE2 and streptavidin particles (blue) show Gaussian-like symmetrical distributions of z heights at the main peak when prepared using the ESI-cryoPrep method. These distributions appear to have the same mean, mode and median, and low absolute skewness value.

DOI