Heatmap of angular orientations of the five protein samples, 70S ribosome (first panel), 20S proteasome (second panel), apo-ferritin (third panel), ACE2 (fourth panel) and streptavidin (fifth panel). Data using the ESI-cryoPrep method are shown on the top panel, while the control data on supporting films using the conventional method are shown on the bottom. AngleRot, AngleTilt are two given Euler angles calculated in RELION.
