Microscopy from Scientific Research

Four-plex DNA-PAINT image of EGF-stimulated EGFR–ALFA–mEGFP transfected CHO cells. Three different binders (anti-GFP-1H1 + 1B2 nanobody (cyan), extracellular anti-EGFR antibody and anti-mouse kappa light chain nanobody (yellow) and intracellular anti-EGFR antibody and anti-rabbit IgG nanobody (red)) with different labeling efficiencies were used. Schematics of the construct for EGFR dimers is shown, with the respective mean labeling efficiency for each target binder (mean). Zoom-ins highlight the EGFR dimerization for each target binder.

DOI