Quantitative analysis as shown in c and d yielded the labeling efficiency of nanobodies against most widely used tags. Data are shown as a box plot, where the median is indicated by the center black line. Boxes extend from the 25th to the 75th percentile of each group’s distribution of values. The whiskers extend to points that lie within 1.5 interquantile range of the lower and upper quartile. Data are of n = 6 (GFP-1H1 and ALFA-tag), n = 7 (mCherry and mNeonGreen) cells over three independent experiments and n = 5 (GFP-1B2, GFP-1H1, GFP-1B2, TagRFP, mEOS2 and SPOT-tag), n = 6 (GFP-1H1, GFP-1B2 and ALFA-tag) over two independent experiments.
