Workflow from Scientific Research

Summary of VR-aided deep learning for antibody-labeled cell segmentation in mouse brains. (i) Fixed mouse brains are subjected to SHANEL-based antibody labeling, tissue clearing and fluorescent light-sheet imaging. (ii) Volumes of raw data are labeled in VR to generate reference annotations. (iii) The DELiVR pipeline was packaged in a Docker container, controlled via a Fiji plugin. DELiVR segments cells using deep learning and registers them to the Allen Brain Atlas. DELiVR produces per-region cell counts and generates visualizations with all detected cells color coded by atlas region.

DOI