Bar Plot from Scientific Research

Validation of NFAT activity screening hits using electroporation of in vitro transcribed sgRNAs coupled with ABE8e protein, followed by alpha-CD3/CD28 stimulation for 16 h and analysis of GFP (NFAT) or cyan fluorescent protein (CFP; NFkB) transcriptional activity reporters. Two sample t -test P values compared with HEK 3 relative color control are shown if P > 0.001; n = 3 activation replicates, where s.d. is shown.

DOI