Relative transcript abundances of genes encoding for reactive oxygen scavenging and ( b ) osmoprotectant synthesis genes. Only genes with a significant change in expression (DeSeq2 adj. p < 0.05) between subsequent time points ( n = 3 independent crust samples per time point) or between experiment phases ( n = 6 independent crust samples in early hydration phase, n = 9 for dry and main hydration phase) are shown. Effect sizes (as Log2-fold change) and BenjaminiHochberg false discovery rate adjusted p values (calculated with DeSeq2) for analyzed genes indicative of discussed metabolisms, can be found in Supplementary Data 3 . Heat map columns depict individual time points of the time series (average values of three replicates), whereas rows depict transcripts attributed to a specific MAG. Taxonomy of individual MAGs are color-coded. Numbers parenthetically indicate the number of genes summarized per MAGs (encoding different subunits or multiple copies of the same gene). The highest color intensity indicates the time point where the respective transcript reached its highest proportion in a MAGs transcriptome. This maximum value is indicated on the right in transcripts per million (TPM) (gray bars). The vertical red line indicates values far exceeding 20,000 TPM.
