Binding curves of Anc4 + Loop II + A + B + C and Anc5ΔLoop I. Binding curves were fitted with plots of the absorbance of the supernatants of each protein samples at 280 nm after pull-down assay. The pull-down assay was performed in 10 mM sodium acetate buffer (pH 5.0) at 25 °C, using 0.1% (w/v) cell wall fraction from T. longibrachiatum as the substrate. Error bars represent the SD of the means ( n = 3).
