Dual-luciferase reporter assay in the UMNSAH/DF-1 cell line demonstrated the potential cis -regulation activity of the 405 bp ERVK solo-LTRs insertion located upstream of the ITGA2 gene in the zebra finch. Relative luciferase activity was determined with n = 9 biologically independent repetitions for each experimental group. Horizontal lines indicate the mean (± s.d.) in ( f , g ). Welch’s two-tailed t-test was conducted using GraphPad Prism ( p -value: **** < 0.0001) in ( f , g ). Source data are provided as a Source Data file.
