The canonical CRE motif bound by ATF-1 is shown, which contains a central CpG site. DNA methylation analysis of annotated CRE motifs in DNMT3L-OE (n= 3) compared with control hTSCs (n= 3) show a significant increase in CpG methylation (chi-squared statistic,p< 0.0001). CRE motifs with a minimum of 10 reads in every sample were included in the analysis (n= 69,819). Methylated is defined as >60% methylation, partially methylated is 20%–60% methylation, and unmethylated is <20% methylation.
