Immunofluorescence images show pCREB and SDC1 in control and DNMT3L-OE hTSC-Orgs. Nuclear counterstaining was with DAPI. Scale bars, 100 μm. Quantification of nuclear pCREB signal from 10 organoids per group is shown. DNMT3L-OE hTSC-Orgs exhibit reduced levels of pCREB, data are presented as mean ± SEM;∗p< 0.05 (Student’s two-tailed t test) (arbitrary units [a.u.]).
