The western blot shows HSP90, DNMT3L, pCREB (Ser133), pATF-1, and total CREB abundance in DNMT3L-OE hTSC-Orgs compared with controls. Quantification of CREB band intensities is shown in the graph on the left. Data are normalized against HSP90 and represented relative to control samples. DNMT3L-OE hTSC-Orgs show reduced protein levels of CREB, data are mean of n= 3 independent experiments ± SEM;∗p< 0.05 (Student’s two-tailed t test).
