Screenshot of mean DNA methylation of 100-CpG windows across 3.7 Mbp region of chromosome 3, in BT-hTSCs, CT-hTSCs, control hTSCs, DNMT3L-OE hTSCs, and 1stCTBs (ns as shown in E), demonstrating increased DNA methylation particularly within PMDs. Annotation tracks are shown for H3K27me3 regions defined in 2ndCTBs or hTSCs, placental partially methylated domains (PMDs) defined in 1stCTBs, and hypermethylated differentially methylated regions (DMRs) in DNMT3L-OE compared with controls.n represents the number of biological replicates per sample, unless otherwise indicated.
