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Schematic of experimental design. The Hu lambs (11 days of age) were separated from their dams and fed mixed goat milk using an artificial nursing bottle. After 3 days of adaptation, 24 lambs were randomly assigned to three groups with the following treatments: normal saline infusion (Con, n = 8), 3-IAld infusion (3-IAld, n = 8), and PGD2 infusion (PGD2, n = 8). At 42 days of age, the lambs were slaughtered and the ruminal epithelium and muscle tissue were collected for analysis.

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