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Schematic of cell culture experimental design. Primary ruminal epithelia cells and smooth muscle cells were isolated from the rumen epithelia and muscular layer of Hu lambs (42 days of age). Primary ruminal epithelia cells were divided into control (n = 5), 3-IAld (n = 5), 3-IAld + AhR inhibitor (n = 5), and 3-IAld + -catenin inhibitor (n = 5) group. To identify the effect of PGD2 on primary ruminal smooth muscle cell proliferation, the cells were treated with DMSO (n = 5), PGD2 without (n = 5) or with CAMK2 inhibitor (n = 5).

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